Proteína codificada por el gen supresor CDKN2 (MST1, INK4a) situado en el cromosoma 9p21, inhibidora de las quinasas dependientes de ciclinas, cdk4 y cdk6, que participan en la regulación de la fase G1 del ciclo celular. La expresión de esta proteína está estrictamente regulada en células normales en las que se expresa a niveles muy bajos, no detectándose mediante inmunohistoquímica. Por el contrario, p16 (INK4a) se encuentra sobreexpresada en la lesión escamosa intraepitelial de cérvix uterino (SIL) relacionada con Papilomavirus humano (HPV). Es especialmente frecuente en lesiones de alto grado (H-SIL), en carcinoma escamoso y en lesiones con cepas de HPV asociadas a alto riesgo de progresión. En SIL de bajo grado la expresión es más heterogénea y el epitelio cervical normal es negativo. La positividad inmunohistoquímica se observa en núcleo y citoplasma y es de utilidad en el diagnóstico diferencial de SIL en biopsias o frotis citológicos.
BIBLIOGRAFÍA
•Hu L. et al. Human papillomavirus genotyping and p16INK4a expression in cervical intraepithelial neoplasia of adolescents. Mod Pathol 2005;18:267-273.
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•Kalof AN. et al. p16INK4a immunoexpression and HPV in situ hibridization signal patterns. Potential markers of high-grade cervical intraepithelial neoplasia. Am J Surg Pathol 2005;29:674-679.
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•Wang JL. et al. p16 INK4A and p14ARF expression pattern by immunohistochemistry in human papillomavirus-related cervical neoplasia. Mod Pathol 2005;18:629-637.
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•Agoff SN. et al. p16 (INK4a) expression correlates with degree of cervical neoplasia: a comparison with Ki-67 expression and detection of high-risk HPV types. Mod Pathol 2003;16(7):665-673.
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•Klaes R. p16 INK4a immunohistochemistry improves interobserver agreement in the diagnosis of cervical intraepithelial neoplasia. Am J Surg Pathol 2002;26(11):1389-1399.
bullet
•Keating JT. et al. Ki-67, cyclin E, and p16 INK4 are complementary surrogate biomarkers for human papiloma virus-related cervical neoplasia. Am J Surg Pathol 2001;25:884-891.
bullet
•Sano T. et al. Expression status of p16 INK4a protein is associated with human papillomavirus oncogenic potential in cervical and genital lesions. Am J Pathol 1998;153:1741-1748.
bullet
•Nakao Y. et al. Introduction of p16 during immortalization by HPV 16 and 18 and not during malignant transformation. Br J Cancer 1997;75:1410-1416.
Fuente: http://www.histopat.es/tec-inm-banco.htm
BIBLIOGRAFÍA
•Hu L. et al. Human papillomavirus genotyping and p16INK4a expression in cervical intraepithelial neoplasia of adolescents. Mod Pathol 2005;18:267-273.
bullet
•Kalof AN. et al. p16INK4a immunoexpression and HPV in situ hibridization signal patterns. Potential markers of high-grade cervical intraepithelial neoplasia. Am J Surg Pathol 2005;29:674-679.
bullet
•Wang JL. et al. p16 INK4A and p14ARF expression pattern by immunohistochemistry in human papillomavirus-related cervical neoplasia. Mod Pathol 2005;18:629-637.
bullet
•Agoff SN. et al. p16 (INK4a) expression correlates with degree of cervical neoplasia: a comparison with Ki-67 expression and detection of high-risk HPV types. Mod Pathol 2003;16(7):665-673.
bullet
•Klaes R. p16 INK4a immunohistochemistry improves interobserver agreement in the diagnosis of cervical intraepithelial neoplasia. Am J Surg Pathol 2002;26(11):1389-1399.
bullet
•Keating JT. et al. Ki-67, cyclin E, and p16 INK4 are complementary surrogate biomarkers for human papiloma virus-related cervical neoplasia. Am J Surg Pathol 2001;25:884-891.
bullet
•Sano T. et al. Expression status of p16 INK4a protein is associated with human papillomavirus oncogenic potential in cervical and genital lesions. Am J Pathol 1998;153:1741-1748.
bullet
•Nakao Y. et al. Introduction of p16 during immortalization by HPV 16 and 18 and not during malignant transformation. Br J Cancer 1997;75:1410-1416.
Fuente: http://www.histopat.es/tec-inm-banco.htm
