Bacterial E.Coli Colony PCR Protocol
Colony PCR Quick Prep Method
·Step 1:
Pick a bacterial colony with an autoclaved toothpick or pipette tip and swirl it into 25 μl of TE autoclaved dH2O in an autoclaved microfuge tube. Do not remove the entire colony, and mark the area you picked preferrably number the bottom of the plate (and the microfuge tube).
·Step 2:
Heat the mix in a boiling water bath or hot (90-100C) heating block for 2 minutes
·Step 3:
Spin sample for 2 minutes high speed in centrifuge.
·Step 4:
Transfer 20 μl of the supernatant into a new microfuge tube.
·Step 5:
Take 1-2 μl of the supernatant as template in a 25 μl PCR standard PCR reaction.
